ABSTRACT
<p><b>OBJECTIVE</b>A 6-month observation on curative effect of Panlongqi Tablet (PLQ) in treating patients with ankylosing spondylitis (AS) was conducted for evaluating its efficacy and safety.</p><p><b>METHODS</b>One hundred and four inpatients and outpatients with AS were randomly assigned to two groups by using random number table, 52 in each group. All patients were treated non-steroid anti-inflammatory drug (NSAID) and sulfasalazine (SSZ), and those in the treated group were given with PLQ additionally. The change of symptoms and signs were observed, and some laboratory indexes as Bath AS disease activity index (BASDAI), Bath AS functional index (BASFI), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), as well as adverse reaction were observed as well. The revisit and follow-up study were carried out at the 3 months and 6 months respectively.</p><p><b>RESULTS</b>The lumbosacral pain was significantly alleviated, the time for lumbodorsal morning stiffness was shortened, the levels of BASDAI, BASFI, ESR and CRP were significantly lowered after treatment for 3 months or 6months (P < 0.05), and these improvements were more significant in the treated group as compared with those in the control group (P < 0.05). The main adverse reactions was dominantly the gastrointestinal symptoms, which showed insignificant difference between the two groups (P >0.05).</p><p><b>CONCLUSION</b>PLQ is an effective traditional medicine for AS with mild adverse reaction, it can be used as an adjuvant therapy.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Anti-Inflammatory Agents, Non-Steroidal , Therapeutic Uses , Drug Therapy, Combination , Drugs, Chinese Herbal , Therapeutic Uses , Phytotherapy , Spondylitis, Ankylosing , Drug Therapy , Sulfasalazine , Therapeutic Uses , Tablets , Treatment OutcomeABSTRACT
<p><b>OBJECTIVES</b>To construct and screen a primarily phage display library of HCV C and E1 genes evolved with an artificial pattern.</p><p><b>METHODS</b>Two genes of about 1 kb with different genotypes were evolved by DNA shuffling. The re-assembled HCV C and E1 genes were cloned into a phage vector. After being rescued with helper phage M13KO7, a phage display library was constructed. Then the library was screened with anti-C and E1 McAb. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was carried out on twenty individual phage clones selected randomly to detect their binding and reactive activity with high-titer HCV-positive sera. Normal sera were used as controls.</p><p><b>RESULTS</b>The phage display library of HCV C and E1 genes which evolved with an artificial pattern was constructed. Their capacity amounted to 1.64 x 10(6), and 86 percent of the clones contained C and E1 genes. After four rounds of panning, the phage library was specifically enriched. Twelve positive clones were successfully screened.</p><p><b>CONCLUSION</b>The capacity and diversity of the constructed library are enough for screening. The results demonstrate the superiority of the specific binding and reactive activity and affinity of the 12 phage clones from the HCV positive sera.</p>
Subject(s)
DNA, Viral , Genetics , Gene Library , Hepacivirus , Genetics , Peptide Library , Viral Core Proteins , Genetics , Viral Envelope Proteins , GeneticsABSTRACT
Objective To evaluate the efficacy and safety of methotrexate(MTX)on the hip involve ment in patients with ankylosing spondylitis(AS).Methods Among the AS patients with hip joint involvement admitted to the department from 1999—2002,50 patients were treated with sulfasalazine(SSZ)(control group, CG)and 48 MTX(observation group,OG).One kind of NSAIDs was taken by the patients in both groups as the basic therapy.The treatment was maintained in both groups after being discharged.Patients were followed-up for three years.The observation parameters included symptoms,signs,Bath AS disease activity index(BASDAI), Bath AS functional index(BASFI),hip joint function score,the CT staging of hip joint involvement,serum in- flammation markers,laboratory tests and side effects.The data were analyzed with SPSS10.0 statistics software. Results At the 1st,2nd and 3rd year,44,38 and 32 cases in the OG and 45,38 and 31 cases in the CG were followed up respectively.During the three-year follow-up period,the hip joint function score of the OG was significantly higher than that of the CG(P